Secretome characterization of the lignocellulose-degrading fungi <i>Pycnoporus sanguineus</i> and <i>Ganoderma resinaceum</i> growing on <i>Panicum prionitis</i> biomass

C4 grasses are common species in rangelands around the world and represent an attractive option for second-generation biofuel production. Although they display high polysaccharide content reach great levels of biomass accumulation, there is a major technical issue to be addressed before can used bioethanol industrial production: lignin removal. Concerning this, Pycnoporus Ganoderma fungal genera have been highlighted due their ability hydrolyze lignocellulose biological pretreatments. Our goals here were evaluate pretreatment efficiency using secretome from spp. harvested glucose-free inductive medium (using grass) identify enzymatic activities responsible degradation glucose release. results show that P. sanguineus exhibits higher activity lignocellulolytic enzymes such as cellulases, xylanases, laccases, manganese peroxidases compared with G. resinaceum. Interestingly, zymograms presence 2 M suggest β-glucosidase isoform could tolerant. The proteomic approach carried out allowed identification 73 180 different proteins resinaceum secretomes, respectively, which functionally classified five main categories miscellaneous group. These open new avenues future experimental work lead deeper comprehension greater application mechanisms underlying lignocellulosic degradation.